AD pig (APP, TAU, PS1) Expression

Our company produces the world’s first six-fold transgenic cloned AD pigs that co-express the APP, TAU and PS1 genes. Compared to mouse models, it is possible to obtain results that are much more similar to humans in the pre-clinical efficacy tests of candidate drugs for AD. The results obtained are the most similar to human trials for these candidate drugs.

  • Gene
    • Presenilin1
    • APP
    • Tau
  • Human-pig amino acid
    sequence similarity (%)
    • 96
    • 99
    • 81
  • Human-mouse amino acid
    sequence similarity (%)
    • 94
    • 99
    • 74
Production of Transgenic Pig as an Alzheimer’ s disease Model Using a Multi-Cistronic Vector System , PLoS One. 2017 Jun
Development of Two Vectors for the Production of AD-tg Pigs
  • pTet-hPDGFb pro-APPsw-2A-TAU-2A-PS1-SV40 pA

    Human-derived promoter comprised multi-vector

  • pTet-porcine Thy1 pro-APPsw-2A-TAU-2A-PS1-SV40 pA

    Pig nerve cell-specific expression multi-vector

Confirmation of AD-tg Pigs Transgene
  • Thy1 Pro-AD transgenic (Tg) pig (♀)
  • Thy1 Pro-AD transgenic (Tg) pig (♂)
Pathological Evaluation of AD-tg Pigs

Frozen section, Coronal section, 10μm thickness, IHC

  • Amyloid β
    • DAPI
    • Aβ specific
    • Merge
  • Amyloid β
    • GFAP
    • GFAP
    • GFAP

PLOSONE (Published, 2017.06.06.)

Biomarker Evaluation of AD-tg Pigs
  • Confirmed expression of Aβ40, Aβ42, Total tau in brains of AD Tg porcine model (around 7 weeks): ELISA

※ Data were analyzed by independent samples t-test in SPSS (*: p < 0.05, **: p < 0.01)

PLOSONE (Published, 2017.06.06.)

Brain PET-CT Data from AD Pig Models
  • Mid-Sagittal
    • WT
    • TG
  • Axial
    • WT
    • TG
  • Coronal
    • WT
    • TG
  • 01
  • SUV
  • 05

If you would like to test the efficacy of a candidate substance at the cellular level, please request a research purpose cell line.

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