Bio-Derived Active Substances
01

Stem cell culture medium can be supplied.

Please contact us for more information on stem cell culture medium.

Stem Cell Culture Medium
  • Human adipose stem cell culture medium is
  • a safe, medical-grade raw material that has been certified by the Korea Testing & Research Institute (KTR) through its raw material stability test.
  • It is a US ICID-listed raw material, and it is free from foreign virus and mycoplasma contamination.
  • It is also free of antibiotics, serum, endotoxins and bacteria, and it has passed the skin patch stability test.
Raw Material Stability
Human adipose conditioned medium with proven safety

(11 categories of medical level testing completed: skin irritation, ocular mucous membrane irritation, photosensitivity, skin sensitization, phototoxicity, single oral administration, single epidermal administration, reverse mutation to microbe, nucleolus, chromosomal abnormality, repeated epidermal administration)

01
Donor eligibility screening
02
Cell/tissue collection and screening
03
Culture facility and management
04
Production of human cell/tissue culture medium
05
Safety test of human cell/tissue culture medium
  • Listed in International Cosmetic Ingredient Dictionary (ICID)
  • Foreign virus, mycoplasma test (cell and tissue stability test passed)
  • Absence of antibiotics test
  • Absence of endotoxin, microbes test
  • Skin patch stability test
Component Analysis
  • Human adipocyte conditioned media (Mirae Bio Stem Secret Adipo BM-1) contains bioactive ingredients such as growth factors, cytokines, proteins, peptides, etc. that are secreted from the cell.
  • Procollagen: promotes the formation of collagen, an important component in the dermal layer of skin, effectively improving the elasticity of skin and wrinkles.
  • Vascular endothelial growth factor (VEGF): revitalizes the skin
  • Fibroblast growth factor (bFGF): promotes the growth of skin cells
  • Insulin-like growth factors: promotes regeneration and growth of tissues
  • Amino acid: skin moisturizing effect
  • Procollagen

    Contains 1,300 ng per 1 ml of procollagen, an effective agent in improving the elasticity of skin and wrinkles by promoting the formation of collagen, which is an important component in the dermal layer of skin.

  • Vascular endothelial growth factor (VEGF)

    Contains 1,500 pg per 1 ml of VEGF, a substance that revitalizes the skin.

Efficacy
  • 01 Anti-Inflammation Efficacy
    • TNF-α: inhibition effect at 0.04-10% concentration (inhibits 30.9-63.0% of production)
    • IL-6: inhibition effect at 0.008-0.04% concentration (inhibits 8.4-16.0% of production)
    • NO: inhibition effect at 0.008-10% concentration (inhibits 6.0-1.2% of production)
  • 02 Wrinkle Improvement
    • MMP-1: inhibition effect at 0.04-10% concentration
  • 03 Moisturizing efficacy
    • Hyaluronic acid: production promoted at 0.2-10% concentration
    • HAS2: expression promoted at 1.25-2.5% concentration
  • 04 Skin regeneration efficacy
    • 1) Cell proliferation promotion (wound-healing efficacy)
    • Cell proliferation promoted at 5-10% concentration
  • 05 Skin regeneration efficacy
    • 2) Promotion of angiogenesis (angiogenesis assay)
    • Promotion of angiogenesis effect in HUVEC cell’s tube formation assay:
      Promotion of angiogenesis at 6.3-50% concentration
02

Cell bank used for mass production of bioactive substances secreted by cells can be supplied.

Please inquire about cell banks used for mass production of exosome.

03

Jeju Black Cattle placenta extract can be supplied.

Please request more information on Jeju Black Cattle placenta extract.

Jeju Black Cattle placenta extract
  • Jeju Black Cattle placenta extract can be supplied.
  • The placenta contains the three major nutrient groups, proteins/carbohydrates/ lipids, along with nutrients and active ingredients such as amino acids and active peptides, various types of vitamins, minerals, enzyme sugars, and nucleic acids.
  • The placenta also contains hepatocyte growth factor (HGF), nerve cell growth factor (NGF), epidermal growth factor (EGF), fibroblast growth factor (FGF), and insulin-like growth factor (IGF), etc., so direct activation of cells can also be expected.
  • The placenta is also known to be especially effective in improving fine wrinkles, deep wrinkles and freckles, preventing aging, regenerating the skin, and whitening the skin.
Efficacy

Jeju Black Cattle Placenta Extract (JBC-PE) has been shown to have statistically significant effects on anti-oxidation, anti-inflammation, anti-atopic dermatitis, wrinkle improvement, moisturization, whitening, and skin regeneration.

  • 01 Antioxidant efficacy
    • Evaluation of ABTS (2,2’-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) free radical elimination activity: Increased activation by 6.1-69.9% at 0.05-1% concentration
    • Evaluation of FRAP (ferric reducing antioxidant power) activation showed reducing power of 8.08mM FSE/solution
  • 02 Anti-inflammation efficacy
    • IL-1α: inhibition effect at 0.006-0.1% concentration (inhibits 30.9-63.0% of production)
    • IL-6: inhibition effect at 0.006-0.05% concentration (inhibits 8.4-16.0% of production)
    • NO: inhibition effect at 0.006-0.025% concentration (inhibits 6.0-1.2% of production)
  • 03 Anti-atopic efficacy
    • IL-6: inhibition effect at 0.006-0.05% concentration (inhibits >22.9% of production)
    • IL-8: inhibition effect at 0.05% concentration (inhibits >55.9% of production)
  • 04 Wrinkle improvement
    • MMP-1: inhibition effect at 0.0003-0.005% concentration (inhibits 19.6-59.2% of production)
    • Elastase: inhibition effect at 0.0006-0.01% concentration (inhibits 13.8-94.9% of production)
  • 05 Moisturizing efficacy
    • Hyaluronic acid: production promoted at 0.006-0.1% concentration (3.9-15.4 times)
    • HAS2: production promoted at 0.05-0.1% concentration (2.2-5.6 times)
    • AQP3: production promoted at 0.006-0.1% concentration (1.7-7.4 times)
  • 06 Whitening efficacy
    • L-DOPA: decreased oxidation activity at >0.005 concentration
    • Inhibition of tyrosinase activity: inhibition at <0.1% concentration
  • 07 Skin regeneration efficacy
    1) Cell proliferation promotion (wound-healing efficacy)

    Proliferation activity evaluation of HaCAT cell, a human keratinocyte: >35% at 0.1% concentration

  • 08 Skin regeneration efficacy
    2) Promotion of angiogenesis (angiogenesis assay)

    Promotion of angiogenesis effect in HUVEC cell’s tube formation assay > 12-45% increase from 0.3-2.5% concentration